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Although the mechanisms underlying age associated muscle loss are not entirely understood, researchers attempted to moderate the loss by increasing the regenerative capacity of muscle. This involved the injection of a recombinant adeno-associated virus directing overexpression of insulin-like growth factor I (IGF-I) in differentiated muscle fibers. They demonstrated that the IGF-I expression promotes an average increase of 15% in muscle mass and a 14% increase in strength in young adult mice (Figure 1), and remarkably, prevents aging-related muscle changes in old adult mice, resulting in a 27% increase in strength as compared with uninjected old muscles (Figure 2). Muscle mass and fiber type distributions were maintained at levels similar to those in young adults. These results suggest that gene transfer of IGF-I into muscle could form the basis of a human gene therapy for preventing the loss of muscle function associated with aging and may be of benefit in diseases where the rate of damage to skeletal muscle is accelerated. Discussion: I?m not sure where to begin. This study has the potential to completely change the way we age. In this experiment, a recombinant adeno-associated virus, directing overexpression of insulin-like growth factor I (IGF-I) in mature muscle fibers, was injected into the muscles of mice. The DNA that was originally in the virus was removed along with markers that stimulate immune response. DNA coding for IGF-1 was then put into the virus along with a promoter gene to ensure high rates of transcription. The results, as you can see by figures 1 & 2, were dramatic. IGF-1 plays a crucial role in muscle regeneration. IGF-1 stimulates both proliferation and differentiation of stem cells in an autocrine-paracrine manner, although it induces differentiation to a much greater degree. IGF-1, when injected locally, increases satellite cell activity, muscle DNA, muscle protein content, muscle weight and muscle cross sectional area. The importance of IGF-1 lies in the fact that all of its apparent functions act to induce muscle growth with or without overload although it really shines as a growth promoter when combined with physical loading of the muscle. IGF-1 also acts as an endocrine growth factor having an anabolic effect on distant tissues once released into the blood stream by the liver. IGF-1 possesses the insulin-like property of inhibiting degradation, but in addition can stimulate protein synthesis. The insulin-like effects are probably due to the similarity of the signaling pathways between insulin and IGF-1 following ligand binding at the receptors. The ability of IGF-I to stimulate protein synthesis resembles the action of GH, which was shown in separate studies on volunteers to stimulate protein synthesis without affecting protein degradation. Although it is often believed that the effects of GH are mediated through IGF-1, this cannot be the case entirely. First, the effects of the two hormones are different, in that GH does not change protein degradation. Second, the effect of GH is observed with little or no change in systemic IGF-1 concentrations. Age related muscle loss has been prevented with GH injections, however it is believed that this is accomplished through IGF-1. The results of this study are similar to other studies where IGF-1 was injected directly into muscle tissue, resulting in increases in size and strength of experimental animals. Using a virus as a genetic vehicle has an advantage over simply injecting the growth factor. The effects of a single viral treatment last significantly longer (months if not years) because the muscle cell itself is constantly overproducing its own IGF-1 from injected DNA. The fact that the IGF-1 produced by the muscle of these mice did not reach the blood stream is interesting. Systemic injections of IGF-1 have not been successful in inducing this kind of anabolic effect in humans. In addition, IGF-1 produced by the liver is genetically different than that produced by muscle tissue. It could be that providing additional DNA for the muscle to produce it?s own IGF-1 is the key to achieving anabolic and rejuvenative effects specifically in skeletal muscle. In this study there was a preferential preservation of type IIb muscle fibers in aging mice. These are the fibers most sensitive to muscle hypertrophy from training and they are also the first fibers to disappear with aging. In the mice receiving the engineered virus, there was also a preservation of the motor neuron, leading to an increase in functional capacity. It is speculated that age related muscle loss is secondary to the loss of neuronal activation of type-II fibers. By preventing the degeneration of typ-II motor units, functional capacity could be maintained into old age. This technique may also serve useful in the prevention of osteoporosis. Further study is necessary to determine wether IGF-1 is having an effect only on muscle fibers or on nervous tissues as well. Finally, it was also exciting to see muscle growth in the young mice who received the injection (15% increase in muscle mass). This means that the injection provided levels of IGF-1 far and above what the muscle normally has access to and not simply a preservation of normal levels. Remember that this was not combined with exercise. The growth of the injected muscles happened even without an extreme mechanical stimulus. The mice were simply allowed to run around as they usually do. Because of these dramatic results, the authors expressed concern about the use of this technique to enhance performance or cosmetic appearance. Research Update is not my personal soap box so I won?t go off on the gender centered hypocrisy of cosmetic enhancement in our society. All we can hope for is that this technique will be used to treat more important diseases such as muscular dystrophy and thereby become somewhat available for other uses as well.
IGF-1 or insulin-like growth factor 1 (brand name: Increlex) is a growth factor which is very closely related to insulin. It carries the same amount of amino acids as insulin and responsible for the anabolic reactions to GH. IGF-1 is an important factor in childhood growth and is highly anabolic in adults. It is also known by the brand name Increlex and the generic name mecasermin.
In the 1970’s, IGF-1 was known as as “Sulfation Factor” and “Nonsuppressible Insulin-like Activity” (NSILA). In the 1980’s, it was known as “Somatomedin C.” The most popular type of IGF-1 available on the Black Market is a longer lasting version (more amino acids in length) known as Long R3 Insulin-like Growth Factor-I or Long R3IGF-I. Lr3igf-1 is more potent than the lesser versions which are no longer available on the black market. Of that type of IGF, there are two types commonly available (Media and Receptor grades, respectively). These last two types of IGF mostly just refer to the purity of what is actually in the bottle.
IGF-1 is released in the liver and binds to the IGF receptors within the cells, which ultimately causes a stimulation of cell growth (both causing new tissue formation and existing tissue growth) and an inhibition of cell death. It is a highly anabolic and anti-catabolic compound. For the athlete or bodybuilder, this had many positive effects: increased nitrogen retention and protein synthesis because it is highly anabolic. IGF-1 (in the presence of sufficient protein) actually promotes growth of new muscle cells, which increases the overall number of cells in the muscle.
IGF protects the neurons of the brain as well as promotes growth of new motor neurons, making it more possible to rapidly learn new skills during its use. IGF-1 is also responsible in connective tissue production, improves collagen formation and aids in cartilage repair. Similarly, it affects the bones by aiding in bone production and repair.
In a study done on young adult mice, a compound responsible for increased secretion of IGF-1 in muscle fibers was administered. There was an average increase of 15% in muscle mass and a 14% increase in strength. When the study was then conducted on adult mice, there was a 27% increase in strength in the injected muscles as compared with non-injected muscles. It was also found to prevent aging of the muscles. Muscle mass and muscle fiber growth were similar to the levels found in young adults. These effects are most likely due to the ability of IGF-1 to activate satellite cells, therefore stimulating muscle rejuvenation (1).
In studies conducted where GH and IGF1 were used together, a greater increase of Lean Body Mass and fat reduction was found than by use with each compound alone (2). Researches also believe that use of testosterone would also increase IGF levels in muscle (3). In a 12 week study on subjects using IGF-1, IGF-1+GH, or GH alone subjects in this study, gained around 3kgs of lean mass, and lost around 2kgs of fat(4) .
The complete human IGF-1 Long R3 IGF-1 is 2-3 times more potent than IGF-1 due to less ability to be made inactive by IGF binding proteins (5) (6).
I'm actually a very big fan of Lr3 IGF-1. For me, I've found that it's had beneficial effects on helping me recover from training injuries and has shown to be very helpful in improving my strength, speed, and performance. I also noted some pretty enhanced muscle building effects and very enhanced fat burning when I've been on IGF…nothing on the level of Anabolic Steroids, but still, the effect was very pronounced.
Most users opt for a dose of about 100mcg/day injected bilaterally in the muscle group just trained, immediately post workout.
I suspect that in the coming years, more and more professional athletes will be using IGF, as it is very difficult to test for, and many have switched over from GH to this compound already.
Anecdotally, IGF seems to stack best with Trenbolone and Testosterone, and there's certainly some synergy between these compounds. Lately, MGF is being added to most IGF protocols.
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